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Overview
The Bio Image Intelligent Quantifier
(IQ) is a software system for analyzing scanned images
of 1-D and 2-D electrophoresis separations, blots, and
colonies/plaques. Using the IQ software you can analyze
gel files obtained from protein, DNA, RNA, or blot
samples and report the data.
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General Features
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Analyze protein, DNA, RNA,
carbohydrate, colony & blot samples
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Analyze wet gels, dry gels,
autorads, blots or photographs
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Import TIFF, JPEG, or BMP
files
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Import from GE (Fuji) or
Molecular Dynamics imagers
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Display intensity in optical
density (OD), GE (Fuji) photo stimulated
luminescence (PSL)
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Name an individual band,
spot, cell, or colony
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Illustrate images with
circles, arrows, boxes, and text; combine an
image with text for publication-quality work
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Generate band, spot, colony
and blot data analysis reports
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Export images and/or analysis
in standard TIFF, JPEG, Postscript®, and ASCII
formats
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Comprehensive on-line User's
Manual
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1-D
Electrophoresis Analysis
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Automatically detect lanes
and find bands
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Find irregularly shaped
bands, i.e., biphasic bands, “smiling bands,” or
saturated bands that are substantially wider
than the rest of the lane
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Vary band detection
sensitivity to optimize performance to gel
characteristics
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Quantify bands with actual
band boundaries based on inflection point
analysis
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User-specified Logarithmic,
Linear, or Reciprocal methods of interpolation
for standards
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Side-by-side lane profile and
band size/quantification comparisons
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2-D Electrophoresis Analysis
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Automatically identify spots
in a user-defined area
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Analyze very complex (10,000
spots) gels
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Quantify spots using 36
radians per spot for accurate and reproducible
quantification data
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Local background for each
spot
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Logarithmic or Linear interpolation of Mw and pI
standards |
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Dot/Slot Blot Analysis
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Dot and Slot Blot samples
from 1 row by 1 column, up to 52 rows by 52
columns
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Automatic quantification of
individual blot cells
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Automatic or manual
background
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Normalize cells to a
specified row, column, or cell
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Concentration standards can
be assigned to user-selected cells
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Colonies/Plaques
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Count colonies in a
user-defined area
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Analyze very complex (10,000
colonies) samples
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Determine local background
for each colony
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